Fluorescent Labeling of Nanometer Hydroxyapatite

材料科学技术（英文）, 2008, 24(2): 187-191.

^1, , ^2, , ^3,

1.Key Laboratory for Ultrafine Materials of Ministry of Education, and Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, China

2.Key Laboratory for Ultrafine Materials of Ministry of Education, and Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, China

3.Key Laboratory for Ultrafine Materials of Ministry of Education, and Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, China

基金项目: 上海市科委基础科研基金(05DJ14005) 国家重点基础研究发展计划(973计划)(2005CCA01000) 国家自然科学基金(20425621)

Fluorescent Labeling of Nanometer Hydroxyapatite

Yuan ZHANG ^1, , Yuan YUAN ^2, , Changsheng LIU ^3,

Keywords： Nano-HAP

A novel surface treatment method using 3-aminopropyltriethoxysilane (AMPTES), was developed to immobilize the fluorescein molecule on nano-HAP (nanometer hydroxyapatite) powders. By pretreating the nano-HAP powders surface with AMPTES, fluorescein, chosen on the basis of the chemical structure of the nano- HAP powders, could be bound to the nano-HAP powders surface. The chemical compositions of nano-HAP before and after being labeled were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS). The morphology, phase composition, and the fluorescence characteristics of the nano-HAP powders with and without staining were also investigated. The FTIR and XPS results revealed that fiuorescein had been successfully immobilized on the surface of AMPTES-bound nano-HAP powders via the acylamide bond formation between the -COOH of fluorescein and the -NH2 of AMPTES. The labeled nano-HAP powders possessed strong fluorescent intensity with a little deviation from the maximum emission wavelength of fluorescein. But the morphology and phase composition had no obvious alteration. Under fluorescence microscopy, the labeled nano-HAP powders., even after 24 h cell incubation, exhibited strong fluorescence.

参考文献

[1]	A Hideki;O Masataka .[J].Rep Inst Med Dent Eng,1993,27:39.
[2]	K Ijntema;W J Heuvelsl;C A Dirix .[J].International Journal of Pharmaceutics,1994,112:215.
[3]	S. H. Zhu;B. Y. Huang;K. C. Zhou;S. P. Huang;F. Liu;Y. M. Li;Z. G. Xue;Z. G. Long .Hydroxyapatite Nanoparticles as a Novel Gene Carrier[J].Journal of nanoparticle research: An interdisciplinary forum for nanoscale science and technology,2004(2/3):307-311.
[4]	S Bisht;G Bhakta;S Mitra;A Maitra .[J].International Journal of Pharmaceutics,2005,288:157.
[5]	S C Zhang;S P Li .[J].J Wuhan Univ Tech -Mater Sci Ed,1996,18:5.
[6]	Q H Xia;Y H Yan .[J].J Wuhan Univ Tech -Mater Sci Ed,1999,21:20.
[7]	A Hideki;O Masataka .[J].Rep Inst Med Dent Eng,1992,26:15.
[8]	Q He;A R Mitchell;S L Johnson .[J].Clinical and Diagnostic Laboratory Immunology,2000,7:899.
[9]	Q He;A Mitchell;T Morcol;S J Bell .[J].Clinical and Diagnostic Laboratory Immunology,2002,9:1021.
[10]	唐胜利,刘志苏,艾中立,郑从义,姚相杰,朱忠超.羟基磷灰石纳米粒子诱导人肝癌细胞凋亡的研究[J].中华实验外科杂志,2004(10):1269.
[11]	Yuan YUAN;Shengli TANG;Hua HONG;Chang-sheng LIU .[J].Chinese Journal of Biomedical Engineering,2005,24:26.
[12]	F Wang;W B Tan;Y Zhang;X P Fan and M Q Wang .[J].Nanotechnology,2006,17:R1.
[13]	J K Liu;Q S Wu;Y P Ding .[J].European Journal of Inorganic Chemistry,2005,20:4145.
[14]	Zhilan YIN;Yuan YUAN;Changsheng LIU .[J].J Func Polymer,2004,17:298.
[15]	N Gultekin;F Tihminlioglu;R Ciftcioglu .Eur Ceram Ⅷ,PTS 1-3[J].Key Engineering Materials,2005,264-268:1953.
[16]	M Rainter;J Abkea;E Schnella;D Scharnweberb .[J].Biomaterials,2006,27:4059.

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Fluorescent Labeling of Nanometer Hydroxyapatite

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