{"currentpage":1,"firstResult":0,"maxresult":10,"pagecode":5,"pageindex":{"endPagecode":5,"startPagecode":1},"records":[{"abstractinfo":"综述了近年来<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>在样品预处理领域中的应用,包括<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>样品预富集同高效液相色谱、毛细管电泳和电色谱的联用,微流控芯片中的<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>萃取以及近年来<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>萃取模式的改进等.引用文献65篇.","authors":[{"authorName":"魏芳","id":"240a0786-99b2-481b-b09c-0e5b5f122b12","originalAuthorName":"魏芳"},{"authorName":"林博","id":"0c0a292c-f11d-4103-b5b2-6e7a89629dec","originalAuthorName":"林博"},{"authorName":"冯钰铸","id":"acbea984-a245-4a99-983f-2aab4ac8b9bf","originalAuthorName":"冯钰铸"}],"doi":"10.3321/j.issn:1000-8713.2007.02.006","fpage":"150","id":"737a635b-3b2a-45f7-bfa6-4fc3f7ab2d8f","issue":"2","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"d8b745ab-1b2a-4163-87bc-64ae3f044ecc","keyword":"<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>","originalKeyword":"整体柱"},{"id":"05ab7f9b-4346-46ff-8222-248e1767f243","keyword":"固相萃取","originalKeyword":"固相萃取"},{"id":"6105f0c7-d7d1-4d7b-8117-a048168a555c","keyword":"高效液相色谱","originalKeyword":"高效液相色谱"},{"id":"9d8301d1-19cc-4ebd-9b52-321961a77759","keyword":"毛细管电泳","originalKeyword":"毛细管电泳"},{"id":"70fc37fd-cbde-4bcf-8f85-c7e795884e96","keyword":"毛细管电色谱","originalKeyword":"毛细管电色谱"},{"id":"9654001b-762f-4ec7-9623-26fff161fda6","keyword":"微流控芯片","originalKeyword":"微流控芯片"},{"id":"94556cc2-1800-4c4a-b396-18219f9a28fe","keyword":"样品预处理","originalKeyword":"样品预处理"}],"language":"zh","publisherId":"sp200702006","title":"<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>在样品预处理中的应用","volume":"25","year":"2007"},{"abstractinfo":"分别用乙二胺、二乙胺、三乙胺将自制的以甲基丙烯酸缩水甘油酯(GMA)为单体、乙二醇二甲基丙烯酸酯(EDMA)为交联剂的<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>修饰为弱、强阴离子交换<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>.考察了该<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>的性能,选择出分离蛋白质(牛血清白蛋白、溶菌酶和谷胱甘肽)的最佳实验条件,并在最佳分离条件下考察了这些蛋白质在<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>上的色谱行为和该<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>对纤维素降解酶的分离纯化情况.实验结果表明,该<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>性能良好,可以实现对纤维素降解酶的快速分离与纯化.同时,实验也证明采用梯度洗脱可以实现对某些蛋白质的分离纯化.","authors":[{"authorName":"张轶华","id":"f915d800-c02e-4c84-bd97-7d185b2a4d01","originalAuthorName":"张轶华"},{"authorName":"杨更亮","id":"057c0340-79e0-4389-a09d-55b207faa0db","originalAuthorName":"杨更亮"},{"authorName":"张小乐","id":"1790c039-5029-47e4-b241-ffb2a62ff7ae","originalAuthorName":"张小乐"},{"authorName":"赵菊敏","id":"dedaf34a-cc73-46f7-99e0-f6718026fdec","originalAuthorName":"赵菊敏"},{"authorName":"蔡丽萍","id":"0fb37439-1dd4-466a-8288-aeb96d438614","originalAuthorName":"蔡丽萍"},{"authorName":"陈义","id":"9922d13e-80d1-415e-94b6-7c78d83d97cb","originalAuthorName":"陈义"}],"doi":"10.3321/j.issn:1000-8713.2005.03.001","fpage":"219","id":"6cd548cd-d542-4abc-b67a-82dcfa7d3f24","issue":"3","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"a335b0b4-c2e7-42a5-8894-d4e2ac360b29","keyword":"<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>","originalKeyword":"整体柱"},{"id":"aa0bda8c-8772-49dd-aa6d-5725f350dc17","keyword":"修饰","originalKeyword":"修饰"},{"id":"9f5a501f-019e-4f70-8d68-d1330dc9bda3","keyword":"离子交换色谱","originalKeyword":"离子交换色谱"},{"id":"d0492ef7-34ad-45f2-80a4-10c97f842bb4","keyword":"纤维素降解酶","originalKeyword":"纤维素降解酶"},{"id":"7efee18d-c7ed-4138-bf0e-d9a5a050cfc0","keyword":"蛋白质","originalKeyword":"蛋白质"},{"id":"82be9bc7-7ff7-49a9-b8b1-5e09f472f9e6","keyword":"分离","originalKeyword":"分离"},{"id":"c8499768-bbc6-463a-a860-cb2c90365fa7","keyword":"纯化","originalKeyword":"纯化"}],"language":"zh","publisherId":"sp200503001","title":"阴离子交换<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>对蛋白质的分离与纯化","volume":"23","year":"2005"},{"abstractinfo":"在常规不锈钢色谱管中以甲基丙烯酸为功能单体,采用原位聚合法制备了(5S,11S)-特罗格尔碱(S-TB)的印迹<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>.考察了流动相中添加不同量的醋酸和水对分离的影响,结合台阶梯度洗脱模式在S-TB<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>上实现了对TB消旋体的快速分离.另外,以碱性单体2-二甲基乙基胺甲基丙烯酸酯(DAMA)为功能单体,在毛细管中采用原位聚合法制备了毛细管分子印迹<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>,用于在毛细管电色谱(CEC)中对消旋体1,1'-联-2-萘酚(BNL)进行手性分离.结果表明,以DAMA为功能单体可以制备其他酸性模板的分子印迹聚合物,从而扩大了分子印迹聚合物(MIP)在CEC分离中的应用范围.","authors":[{"authorName":"欧俊杰","id":"8bc68521-7778-4005-83f0-42bf8d598baa","originalAuthorName":"欧俊杰"},{"authorName":"董靖","id":"3a570485-8310-4cf1-ac3d-5d5e30ffc235","originalAuthorName":"董靖"},{"authorName":"吴明火","id":"925d68f6-44d2-4d9d-b6a7-efbaba4ae65d","originalAuthorName":"吴明火"},{"authorName":"孔亮","id":"08a49fb0-cbce-4afe-a510-cc68781b9638","originalAuthorName":"孔亮"},{"authorName":"邹汉法","id":"638953e2-3ba6-43f3-9830-be5b6c5f0a45","originalAuthorName":"邹汉法"}],"doi":"10.3321/j.issn:1000-8713.2007.02.003","fpage":"129","id":"306f3e63-e400-405a-abf2-9062004a05cb","issue":"2","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"93806867-b210-4f38-94a2-55cc67f49941","keyword":"分子印迹","originalKeyword":"分子印迹"},{"id":"508e9840-faad-4c66-a633-26b9f06b8d9b","keyword":"<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>","originalKeyword":"整体柱"},{"id":"73e10c3f-cff9-4b3b-8dad-1bb5e3d25909","keyword":"高效液相色谱","originalKeyword":"高效液相色谱"},{"id":"416e9a83-c430-4011-9311-bd68ab3ff487","keyword":"电色谱","originalKeyword":"电色谱"},{"id":"6e9e8858-4259-4fe9-a65e-2209add004ba","keyword":"手性分离","originalKeyword":"手性分离"},{"id":"2d84a378-707e-4c38-96b2-aaf1fec24220","keyword":"特罗格尔碱","originalKeyword":"特罗格尔碱"},{"id":"6310a932-c59e-4e46-b19d-5197ec19f383","keyword":"1,1'-联-2-萘酚","originalKeyword":"1,1'-联-2-萘酚"}],"language":"zh","publisherId":"sp200702003","title":"分子印迹<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>在高校液相色谱和电色谱手性分离中的应用","volume":"25","year":"2007"},{"abstractinfo":"以药物烟酰胺为模板分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,甲苯和正十二醇的混合溶液为致孔剂,采用原位聚合法制备了具有特定识别性能和分离能力的分子印迹聚合物,并将其作为高效液相色谱固定相,实现了模板分子与烟酸在2 min内的快速分离.在规格为50 mm×4.6 mm i.d.色谱<span style=\"color:red\">柱</span>上,以纯水为流动相(流速为7.0 mL/min)、操作温度为室温的色谱条件下,模板分子与烟酸的分离度达1.8.讨论了流动相中有机溶剂含量、醋酸及碱含量和流速对分离的影响.结果表明,原位聚合法制备的<span style=\"color:red\">整体</span>分子印迹聚合物在以纯水作流动相时对模板分子与其类似物有快速分离能力,这对于体内药物的分离富集研究具有很好的应用前景.","authors":[{"authorName":"李志伟","id":"e9023357-ef0a-4dd2-b08f-815ebaee8981","originalAuthorName":"李志伟"},{"authorName":"刘树彬","id":"3dbb1953-41a2-4c77-ad4e-529394462797","originalAuthorName":"刘树彬"},{"authorName":"杨更亮","id":"8238b8b9-f42c-48e7-a844-c4e8602f4076","originalAuthorName":"杨更亮"},{"authorName":"李保芝","id":"2ce5af3f-aae9-45ab-93ff-b183280d2863","originalAuthorName":"李保芝"},{"authorName":"陈义","id":"5897e67c-95fc-439d-9651-06444e817021","originalAuthorName":"陈义"}],"doi":"10.3321/j.issn:1000-8713.2005.06.010","fpage":"622","id":"758e3151-1291-4fbf-b018-6b10e76ba3fb","issue":"6","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"c0ad60d3-874c-4f92-956f-9f3434b50dc3","keyword":"分子印迹聚合物","originalKeyword":"分子印迹聚合物"},{"id":"45d94ea4-a7b9-4080-ad84-b5058120fca2","keyword":"<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>","originalKeyword":"整体柱"},{"id":"8748bc33-e3be-487a-98ee-d8ece78fc1aa","keyword":"快速分离","originalKeyword":"快速分离"},{"id":"cfea9ed7-ed4d-4911-87d8-b841dffe6890","keyword":"烟酰胺","originalKeyword":"烟酰胺"},{"id":"317e3473-ef9e-4611-8562-2212bd3ad6b9","keyword":"烟酸","originalKeyword":"烟酸"}],"language":"zh","publisherId":"sp200506010","title":"分子印迹<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>快速分离烟酰胺及烟酸","volume":"23","year":"2005"},{"abstractinfo":"综述了近几年来离子交换<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>材料(包括涂敷型和键合型)的研究进展,重点介绍其制备方法和在环境及生物领域中的应用,分析了离子交换<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>目前存在的问题,展望了其将来的发展方向.引用参考文献50篇.","authors":[{"authorName":"黄晓佳","id":"51ba7f10-4e5c-4a15-9a14-3dcc9047abbc","originalAuthorName":"黄晓佳"},{"authorName":"黄本立","id":"eca08d0f-0972-454f-bbd1-79750334dfac","originalAuthorName":"黄本立"}],"doi":"10.3321/j.issn:1000-8713.2007.03.002","fpage":"298","id":"2b9c7d69-db60-42a4-a558-bc170633ed14","issue":"3","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"187e54a0-29ab-484f-8153-514b11cce8ed","keyword":"离子色谱","originalKeyword":"离子色谱"},{"id":"5afa7283-4457-4cd0-b640-93a5429fa041","keyword":"<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>","originalKeyword":"整体柱"},{"id":"cfa7c450-9955-4967-82d3-54d45acc9441","keyword":"综述","originalKeyword":"综述"}],"language":"zh","publisherId":"sp200703002","title":"离子交换<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>材料的研究进展","volume":"25","year":"2007"},{"abstractinfo":"采用溶胶-凝胶法,以四乙氧基硅烷和苯基三乙氧基硅烷作为反应单体,通过酸碱两步催化在毛细管中进行原位缩聚反应,制备了新型有机-无机杂化硅胶基质毛细管<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>,制备过程简单.<span style=\"color:red\">整体</span>柱基质中均匀分布的苯基基团可直接用于反相毛细管电色谱的分离,因而不需要对基质再进行衍生化.优化了<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>的制备条件,采用扫描电镜和压汞法对<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>的微观结构和孔径分布进行了表征.分别考察了溶胶-凝胶初始反应液中水的用量对<span style=\"color:red\">柱</span>床结构的影响和两种单体的配比对材料孔径分布的影响.研究了稠环芳烃类化合物在<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>上的保留行为,用所制备的<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>分离了7种苯酚类化合物,平均<span style=\"color:red\">柱</span>效达100 000塔板/m.","authors":[{"authorName":"严丽娟","id":"54d2c4cf-b0fa-41d8-b46b-e2b9ab8f1c49","originalAuthorName":"严丽娟"},{"authorName":"张庆合","id":"218e0e60-4a1f-4046-82eb-71486cbcc159","originalAuthorName":"张庆合"},{"authorName":"李彤","id":"ff9eedc7-2873-405c-8cdd-6378e8f39ae4","originalAuthorName":"李彤"},{"authorName":"张维冰","id":"94da2511-bdd5-4e80-abae-89821d3844c2","originalAuthorName":"张维冰"},{"authorName":"冯钰锜","id":"58cfce44-6b14-4d8c-b9bc-b92a04ebe9a7","originalAuthorName":"冯钰锜"},{"authorName":"张丽华","id":"6d61bf09-2677-4603-9950-b6d299a2da40","originalAuthorName":"张丽华"},{"authorName":"张玉奎","id":"4eff2168-3bbb-4cc2-a1f4-b73065e5e189","originalAuthorName":"张玉奎"}],"doi":"10.3321/j.issn:1000-8713.2005.05.013","fpage":"499","id":"41886be4-1ce8-4bbf-a8e7-c0fa8ca5d484","issue":"5","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"935823a2-f35c-4624-9e2e-fd6de9944654","keyword":"<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>","originalKeyword":"整体柱"},{"id":"5a3b3b79-2c83-4682-ae88-b49752ae6c01","keyword":"毛细管电色谱","originalKeyword":"毛细管电色谱"},{"id":"bfd7b851-d4a7-4548-ba55-afe56e5fb38b","keyword":"溶胶-凝胶法","originalKeyword":"溶胶-凝胶法"},{"id":"e9f4cbec-9e39-45a6-a671-8afa0e3a5892","keyword":"有机-无机杂化材料","originalKeyword":"有机-无机杂化材料"}],"language":"zh","publisherId":"sp200505013","title":"有机-无机杂化硅胶基质<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>的制备及其电色谱性能","volume":"23","year":"2005"},{"abstractinfo":"近年来,高效液相色谱有机聚合物<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>以其独有的优势在分离大分子物质中得到了广泛的应用和发展.本文结合本实验室的研究工作,并参考有一定影响力的相关文献,综述了近几年来有机聚合物<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>的特征及其在生物大分子分离应用中的新进展.","authors":[{"authorName":"白立改","id":"73ce60f0-4fcf-41d4-954a-ec54ccdda382","originalAuthorName":"白立改"},{"authorName":"牛文敬","id":"09ac8034-68ab-40c3-a42a-4d11dbed814b","originalAuthorName":"牛文敬"},{"authorName":"杨更亮","id":"9f7fc9c2-f215-47df-bb1d-c4e050f8b2cc","originalAuthorName":"杨更亮"}],"doi":"10.3724/SP.J.1123.2013.01047","fpage":"303","id":"f7f323b9-582b-4b60-b677-4a3e543b096a","issue":"4","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"056871a7-1037-4d91-9cdf-5a0e61003c24","keyword":"聚合物","originalKeyword":"聚合物"},{"id":"b27e7634-c02c-4d40-9131-13f9723b7c3e","keyword":"<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>","originalKeyword":"整体柱"},{"id":"17178744-a202-4cff-98f3-bab51ca4c6d6","keyword":"生物大分子","originalKeyword":"生物大分子"},{"id":"f30203a5-24a9-435f-b635-0d378f1d44f1","keyword":"高效液相色谱","originalKeyword":"高效液相色谱"},{"id":"e8a6b801-b47c-467e-a36d-310b53c68fea","keyword":"综述","originalKeyword":"综述"}],"language":"zh","publisherId":"sp201304004","title":"聚合物<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>在生物大分子分离中的应用","volume":"31","year":"2013"},{"abstractinfo":"以4-乙烯基苯硼酸(VPBA)为功能单体,季戊四醇三丙烯酸酯(PETA)为交联剂,乙二醇和二甘醇为二元致孔剂,通过热引发自由基聚合反应,在毛细管内原位聚合制备得到一种poly(VPBA-co-PETA)毛细管<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>.所制备的<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>具有典型的硼酸亲和特性,可以特异性地捕获含有顺二羟基的化合物.在前期实验的基础上,将其用于两种中草药——蒲公英和刺果卫矛中含顺二羟基的小分子活性物质的富集.这两种中药提取物直接用高效液相色谱进行检测时,其活性成分绿原酸等因含量太低而使检测困难或无法检测;经过硼酸亲和<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>富集后收集洗脱液进行检测,色谱峰响应则显著提高.该结果表明所制备的poly(VPBA-co-PETA)<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>可以作为富集中药提取物中含顺二羟基活性物质的有效手段.","authors":[{"authorName":"胡青红","id":"4d6df4ed-5cb2-47c8-aa78-150e0821856a","originalAuthorName":"胡青红"},{"authorName":"王超然","id":"c7ffd8fd-deae-4860-b3ff-6709eea3d034","originalAuthorName":"王超然"},{"authorName":"李静","id":"6d46ebbb-b208-4ea8-9afc-09ff42b1ac05","originalAuthorName":"李静"},{"authorName":"王彦","id":"0513c619-201f-4a7a-870c-7c3b07b8689b","originalAuthorName":"王彦"},{"authorName":"谷雪","id":"caab133d-22f3-41a4-99d8-66aea7256dda","originalAuthorName":"谷雪"},{"authorName":"阎超","id":"56a5990b-9e49-4abe-af0a-3fc1e4855fe2","originalAuthorName":"阎超"}],"doi":"10.3724/SP.J.1123.2012.12040","fpage":"714","id":"708b481a-a642-4246-9380-0df0301fc690","issue":"7","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"09bfd947-db82-4a52-860c-457a6a3fda36","keyword":"硼酸亲和","originalKeyword":"硼酸亲和"},{"id":"260b943b-a307-435f-b372-f1aada721948","keyword":"<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>","originalKeyword":"整体柱"},{"id":"efc30a68-3fbe-4a4a-9abb-1171b4b92283","keyword":"顺二羟基化合物","originalKeyword":"顺二羟基化合物"},{"id":"17c85d31-fb62-473c-87bc-56239a422043","keyword":"活性物质","originalKeyword":"活性物质"},{"id":"a5a403d7-254b-4f29-b6ad-28247c4f38e5","keyword":"中药","originalKeyword":"中药"},{"id":"a7913ee0-4dbb-408f-909b-f725c89c3d6d","keyword":"富集","originalKeyword":"富集"}],"language":"zh","publisherId":"sp201307018","title":"硼酸亲和<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>在富集中药活性物质中的应用","volume":"31","year":"2013"},{"abstractinfo":"<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>是通过在<span style=\"color:red\">柱</span>管内原位聚合或固化的方法制备得到的具有多孔结构的<span style=\"color:red\">整体</span>棒状固定相,与传统的填充<span style=\"color:red\">柱</span>相比,具有通透性好、传质速率快、容易制备等优点,因此在分离分析领域特别是生物分离分析中发挥的作用日益增大.<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>的制备及应用近年来也得到了快速发展,层出不穷的新型<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>已被广泛用于色谱高效分离分析、固相萃取及酶反应器等方面,大大推动了分离分析科学的发展.本文主要总结了近五年来<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>的制备技术及其在蛋白质组学应用中的一些最新研究进展.","authors":[{"authorName":"邹汉法","id":"587b3086-0701-444d-b7ed-d739e466d1f3","originalAuthorName":"邹汉法"},{"authorName":"吴明火","id":"b32285f7-2044-40a7-8467-7553e371f4ca","originalAuthorName":"吴明火"},{"authorName":"王方军","id":"57222334-a6ff-4347-9f3a-bc6ae95ff8a0","originalAuthorName":"王方军"},{"authorName":"吴仁安","id":"6e54ddeb-4975-449b-a397-213d3e1611c6","originalAuthorName":"吴仁安"},{"authorName":"叶明亮","id":"b9359512-4012-4ee3-bcc1-2194dc73b5a1","originalAuthorName":"叶明亮"}],"doi":"10.3321/j.issn:1000-8713.2009.05.003","fpage":"526","id":"b7f02cca-343f-404b-b0a2-12c60c4da3b0","issue":"5","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"97a824b7-be37-4a55-9110-ab58923911e5","keyword":"<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>","originalKeyword":"整体柱"},{"id":"c704966c-f1ab-47cf-a1e0-064ffe3b8be6","keyword":"制备","originalKeyword":"制备"},{"id":"7e79f0c7-1b9b-41e7-aa52-e89f2c4113ae","keyword":"色谱分离","originalKeyword":"色谱分离"},{"id":"b66109dd-8ed9-4c8f-bf84-d71aa861e5c7","keyword":"蛋白质组学","originalKeyword":"蛋白质组学"},{"id":"48a27972-8495-43b4-b4c0-5a9d558d4fd9","keyword":"综述","originalKeyword":"综述"}],"language":"zh","publisherId":"sp200905003","title":"<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>制备技术的新进展及其在蛋白质组学中的应用","volume":"27","year":"2009"},{"abstractinfo":"硼亲和<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>是一种能对顺式二羟基化合物进行选择性分离的重要色谱介质.然而,<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>微结构在制备过程中难以控制.本文以分子结构高度对称的三聚氰胺和三(2,3-环氧基丙基)异氰酸酯(TEPIC)作为共聚试剂,利用“团队硼亲和”原理,采用原位聚合的方法制备了一种新型硼亲和<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>.“团队硼亲和”的作用使该硼亲和<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>对顺式二羟基化合物的结合pH降低至中性范围.结构高度对称的共聚试剂有效地提高了<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>材料的比表面积,使其达80.3 m2/g,显著高于文献中报道的其它硼亲和<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>.","authors":[{"authorName":"刘云春","id":"9226a819-fef1-4470-a62f-2568f5a28468","originalAuthorName":"刘云春"},{"authorName":"刘震","id":"e483abe3-917e-4ae7-8d16-e08192ce737b","originalAuthorName":"刘震"}],"doi":"10.3724/SP.J.1123.2013.02014","fpage":"348","id":"fcf78f2a-b82c-4a0e-8bd2-ac90fa7b92b9","issue":"4","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"4dfe0ccf-7fce-492e-8415-eb363c7a6942","keyword":"硼亲和","originalKeyword":"硼亲和"},{"id":"4058eed7-9ab1-49f2-96ef-d485a8aa4499","keyword":"<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>","originalKeyword":"整体柱"},{"id":"4d721b37-42c4-4e45-b5d6-615cb9edd27d","keyword":"制备","originalKeyword":"制备"},{"id":"be5d5e9b-7f50-43f8-bceb-c173cd184be0","keyword":"顺式二羟基化合物","originalKeyword":"顺式二羟基化合物"},{"id":"1200a313-cd99-4f04-b43f-d26824bfa6eb","keyword":"比表面积","originalKeyword":"比表面积"}],"language":"zh","publisherId":"sp201304011","title":"利用高度结构对称的共聚试剂制备高比表面积的硼亲和<span style=\"color:red\">整体</span><span style=\"color:red\">柱</span>","volume":"31","year":"2013"}],"totalpage":473,"totalrecord":4722}