Chenming XU
,
Hongming GAO
,
Guangjun ZHANG
,
Lin WU
材料科学技术(英文)
An arc light radiation phenomenon in TIG welding process was studied through experiments and theoretical analysis. The arc spectra were acquired under a variety of welding parameters from 200 nm to 1000 nm wavelength range in TIG. The influence of welding parameters on the arc radiation was discussed. The radiation energy from lines emission mechanisms was calculated and the comparison was made with the one from the continuum emission mechanisms. The result shows that the radiation energy from the line emission mechanisms is equal to the one from the background. Based on the experiments and analysis, a physical model of arc light radiation has been developed. In this model, the arc plasma was presumed to be in local thermal equilibrium (LTE) with light thinness property. The Bolzmann distribution and Saha equation were applied to establish the arc light radiation model. This helps lay the foundation for further mathematical modeling of the arc light radiation.
关键词:
Spectral diagnosis
,
null
,
null
,
null
刘志伟
,
朱明睿
,
翟琳辉
,
谭敏佳
色谱
doi:10.3724/SP.J.1123.2016.04040
组蛋白翻译后修饰是一种表观遗传学修饰,参与调控细胞的新陈代谢等重要生理过程.蛋白质组学发展迅速,使监控组蛋白翻译后修饰的动态变化成为可能.目前主要有3种无标定量方法(谱图计数法、峰面积积分法和信号强度法),但何种定量方法更可靠尚未见系统性的详细报道.在稳定同位素标记细胞培养技术(SILAC)基础上,对去乙酰化酶抑制剂(SAHA)调控细胞乙酰化修饰水平的定量数据进行对比,比较3种无标定量方法对组蛋白翻译后修饰进行的定量分析,利用定量结果的标准差(SD)评估定量的可靠性,最终发现基于峰面积积分法定量的结果可靠性最高.该研究对难以进行同位素标记实验的样本分析,尤其对临床样本、大样本的组蛋白修饰谱分析具有重要参考意义.
关键词:
组蛋白翻译后修饰
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无标定量
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可靠性