邹琴
,
张利
,
左奕
,
王华楠
,
李玉宝
,
李小玉
功能材料
以纳米羟基磷灰石/壳聚糖(n-HA/CS)复合材料为原料,以适量的氧化钙和碳酸钙为促凝剂,选用适当的固化液,加入具有促进骨再生和愈合的中药--黄芪多糖(APS),制备了一种新型复合骨水泥,并对其固化时间,抗压强度,表面形貌和细胞相容性进行了分析和表征.结果表明,当黄芪多糖加入量由1%(质量分数)增加到5%(质量分数)时,复合骨水泥的初凝时间由(18.39±0.35)min延长到(28.55±0.96)min,而抗压强度由(15.97±0.92)MPa降至(10.55±0.33)MPa.体外药物释放结果表明,栽药骨水泥在pH=7.4的37℃模拟体液中可持续释放黄芪多糖4w以上.细胞可在其表面附着繁殖,体外细胞相容性良好.
关键词:
羟基磷灰石
,
壳聚糖
,
黄芪多糖
,
复合骨水泥
王惠
,
辛华夏
,
蔡剑锋
,
李芳冰
,
金郁
,
傅青
色谱
doi:10.3724/SP.J.1123.2016.03006
多糖是黄芪的重要成分，但多糖相对分子质量大、极性强，难以用色谱方法直接分析，导致目前缺乏能够反映黄芪多糖组成差异的质量评价方法。首先通过部分酸水解方法，将多糖水解成可供分析的寡糖，建立基于部分酸水解-亲水作用色谱的黄芪多糖指纹图谱。通过正交实验选取最佳水解条件：温度80℃、酸浓度1.5 mol／L，水解时间4 h。该方法重复性好，对20批黄芪药材的多糖指纹图谱分析显示相似度为0.258~0.949，反映出黄芪多糖组成的明显差异。同时建立了黄芪的反相液相色谱指纹图谱，用于控制除多糖以外的其他成分，对同样的20批黄芪药材进行分析，实现对黄芪全面的质量评价。实验表明，基于部分酸水解-亲水作用色谱的黄芪多糖指纹图谱可对黄芪多糖的质量进行有效评价，是对黄芪质量评价方法的重要补充。
关键词:
部分酸水解
,
亲水作用色谱
,
黄芪多糖
,
指纹图谱
,
质量评价
常立娜
,
屈树新
,
林孙忠
,
段可
,
翁杰
无机材料学报
doi:10.3724/SP.J.1077.2011.00022
室温湿法合成载不同浓度黄芪多糖(APS)的羟基磷灰石(HA/APS),通过X射线衍射(XRD)表征APS对HA晶体结构、结晶度及晶粒尺寸的影响;用透射电子显微镜(TEM)表征HA和HA/APS的晶体形貌,用激光粒度仪和比表面积仪分别检测HA的粒度及比表面积;体外培养MC3T3-E1成骨细胞,进行Alamar Blue、噻唑蓝(MTT)及碱性磷酸酶(ALP)检测,光镜观察细胞形貌,研究APS对成骨细胞作用的有效浓度范围及HA/APS对成骨细胞活性及分化的影响.结果表明:所载APS对HA晶体结构、结晶度和晶粒尺寸没有明显影响,晶体形貌没有变化,HA平均粒径为1.17μm,比表面积为132.194 m2/g;APS对成骨细胞的作用呈剂量和时间依赖关系,80～200 μg/mL促进细胞活性及ALP表达,HA/APS增强细胞活性,细胞形貌完整.因此,0.5 g HA载入100～250μg APS时明显促进成骨细胞活性,HA/APS具有作为临床骨缺损填充材料的潜能.
关键词:
羟基磷灰石
,
黄芪多糖
,
生物学性能
Hui WANG
,
Huaxia XIN
,
Jianfeng CAI
,
Fangbing LI
,
Yu JIN
,
Qing FU
色谱
doi:10.3724/SP.J.1123.2016.03006
Astragalus polysaccharides (APS) are important components in Astragalus membranaceus (A. membranaceus). However, for the huge relative molecular mass, strong polarity, and difficulty in chromatographic separation, there's a lack of quality control finely reflecting the composition of APS up to now. To solve the problems, the key lies in degradation of polysaccharides and analysis of the hydrolyzates. A partial acid hydrolysis-hydrophilic interaction liquid chromatography (HILIC) fingerprint for APS and a comprehensive quality evaluation method for A. membranaceus combining with the traditional RPLC fingerprint were developed. Orthogonal experiments were designed to investigate the influencing factors of partial hydrolysis and the conditions were optimized based on HILIC analysis. The hydrolyzates were successfully separated using HILIC-evaporative light scattering detection (ELSD) method and the optimal conditions of hydrolysis were selected as 80℃, 1.5 mol/L trifluoroacetic acid and 4 h, which proved to be stable and repeatable after the validation. This method was applied for the fingerprint analysis of 20 batches of A. membranaceus. Cosine method was used for similarity calculation using the average of samples Nos. 1-10 as reference. Moreover, RPLC fingerprint analysis was processed on other components and the data were compared for comprehensive evaluation. The similarities of ten samples with the reference ranged from 0.258 to 0.949, which were consistent with the chromatograms reflecting the apparent disparity in APS, and could be as a supplement of the RPLC fingerprint. The new method, with the advantages of intuition, convenience, and quantifiable in similarity, could reflect the differences of the composition of polysaccharides after degradation and was successfully applied in profiling of APS, contributing to the quality control together with the RPLC fingerprint.
关键词:
partial acid hydrolysis
,
hydrophilic interaction liquid chromatography (HILIC)
,
Astragalus polysaccharides (APS)
,
fingerprint
,
quality evaluation
