Jin Shujing
,
Ren Ling
,
Yang Ke
材料科学技术（英文）
doi:10.1016/j.jmst.2016.06.022
Although being an essential trace element required for human body health, Cu has long been seriously considered toxic when its amount exceeds certain limitation, which significantly limited the wide application of Cu in biomaterials. However, more and more bio-functions and benefits of Cu were found and confirmed, attracting the attention from biomaterials researchers in recent years. People have tried to immobilize Cu into biomaterials by various ways, in order to develop novel bio-functional Cu containing biomaterials with better bio-adaptions, and several different bio-functions of them have been demonstrated. This paper makes a review of the development of novel bio-functional Cu containing biomaterials, and focuses on their unique roles in enhancing bio-adaption of biomedical materials, including antibacterial performance, stimulating angiogenesis, promoting osteogenesis and inhibition of in-stent restenosis, aiming at proposing a prospective development direction for biomedical materials with better bio-adaptions.
关键词:
Biomaterials
,
Cu
,
Antibacterial
,
Angiogenesis
,
Osteogenesis
,
In-stent restenosis
Chen Shaoming
,
Gao Manman
,
Zhou Zhiyu
,
Liang Jiabi
,
Gong Ming
,
Dai Xuejun
,
Liang Tangzhao
,
Ye Jiacheng
,
Wu Gang
,
Zou Lijin
,
Wang Yingjun
,
Zou Xuenong
材料科学技术（英文）
doi:10.1016/j.jmst.2016.08.001
Although cartilage tissue engineering has been developed for decades, it is still unclear whether angiogenesis was the accompaniment of chondrogenesis in cartilage regeneration. This study aimed to explore the process of anti-angiogenesis during cartilage regenerative progress in cartilage repair extracellular matrix (ECM) materials under Hypoxia. C3H10T1/2 cell line, seeded as pellet or in ECM materials, was added with chondrogenic medium or DMEM medium for 21 days under hypoxia or normoxia environment. Genes and miRNAs related with chondrogenesis and angiogenesis were detected by RT-qPCR technique on Days 7, 14, and 21. Dual-luciferase report system was used to explore the regulating roles of miRNAs on angiogenesis. Results showed that the chondrogenic medium promotes chondrogenesis both in pellet and ECM materials culture. HIF1α was up-regulated under hypoxia compared with normoxia (P?<?0.05). Meanwhile, hypoxia enhanced chondrogenesis. miR-140-5p exhibited higher expression while miR-146b exhibited lower expression. The chondrogenic phenotype was more stabilized in the ECM materials in chondrogenic medium than DMEM medium, with lower VEGFα expression even under hypoxia. Dual-luciferase report assays demonstrated that miR-140-5p directly targets VEGFα by binding its 3′-UTR. Taken together, chondrogenic cytokines, ECM materials and hypoxia synergistically promoted chondrogenesis and inhibited angiogenesis. miR-140-5p played an important role in this process.
关键词:
Biomaterials
,
Bio-adaptation
,
Hypoxia
,
Chondrogenesis
,
Angiogenesis
,
miRNAs
