{"currentpage":1,"firstResult":0,"maxresult":10,"pagecode":5,"pageindex":{"endPagecode":5,"startPagecode":1},"records":[{"abstractinfo":"为提高蛋白质组定量分析的准确度、通量和自动<span style=\"color:red\">化</span>程度，构建了由微升级混合离子交换色谱、<span style=\"color:red\">亲</span><span style=\"color:red\">水</span><span style=\"color:red\">型</span><span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span><span style=\"color:red\">反应器</span>（hIMER）和纳升级反相色谱-电喷雾串级质谱（nanoRPLC-ESI-MS / MS）组成的集成化蛋白质定量分析平台。该平台实现了二甲基<span style=\"color:red\">化</span>标记蛋白质样品在线分离、<span style=\"color:red\">酶</span>解、肽段分离鉴定和定量分析。采用质量比为1：1的轻、重标记的蛋白质样品考察该平台的定量性能，发现蛋白质水平二甲基<span style=\"color:red\">化</span>标记效率为90％；蛋白质经 hIMER 在线<span style=\"color:red\">酶</span>解10 min 产生的漏切及<span style=\"color:red\">酶</span>解产物在 hIMER 柱上的非特异性吸附对定量准确度的影响较小，所有定量到的重/轻标记的蛋白质质量比的平均值为1.01。最后将该平台应用于小鼠腹水<span style=\"color:red\">型</span>肝癌淋巴道高、低转移细胞系差异蛋白质的分析，发现了12种蛋白质在高转移细胞系中低表达，15种蛋白质在高转移细胞系中高表达。以上结果证明了该平台可以实现高准确度和高通量的蛋白质组定量分析。","authors":[{"authorName":"周愿","id":"c7c85059-71ac-4ca6-b6df-5aa98d7da6a7","originalAuthorName":"周愿"},{"authorName":"张珅","id":"906b1e6c-9bc6-46c1-82dc-ecc363f3f565","originalAuthorName":"张珅"},{"authorName":"袁辉明","id":"83e63d87-e27b-47d9-b7d8-0d560a1b3a81","originalAuthorName":"袁辉明"},{"authorName":"张丽华","id":"fbd1ee0f-f2a7-4109-ad20-68cd8a780722","originalAuthorName":"张丽华"},{"authorName":"张玉奎","id":"86343eaf-144b-489d-a7b4-23dc32d1e597","originalAuthorName":"张玉奎"}],"doi":"10.3724/SP.J.1123.2014.01002","fpage":"355","id":"e332831f-fb92-442f-9570-84de49f0c422","issue":"4","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"872756c9-3029-46d5-b0bc-0aaf1d4a2f85","keyword":"集成化蛋白质组定量分析平台","originalKeyword":"集成化蛋白质组定量分析平台"},{"id":"03489836-48c5-46bb-af5d-35161a742b4b","keyword":"蛋白质标记","originalKeyword":"蛋白质标记"},{"id":"2107866c-6991-4648-89be-f278e25e0a52","keyword":"定量蛋白质组学","originalKeyword":"定量蛋白质组学"},{"id":"a5d01996-5cd9-4e30-8428-4d43a3b99150","keyword":"<span style=\"color:red\">亲</span><span style=\"color:red\">水</span><span style=\"color:red\">型</span><span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span><span style=\"color:red\">反应器</span>","originalKeyword":"亲水型固定化酶反应器"}],"language":"zh","publisherId":"sp201404006","title":"集成化蛋白质组定量分析平台的构建及应用","volume":"","year":"2014"},{"abstractinfo":"<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span><span style=\"color:red\">反应器</span>作为蛋白质组学研究中“bottom-up”策略重要的组件，具有<span style=\"color:red\">酶</span>解快速、<span style=\"color:red\">酶</span>解效率高、<span style=\"color:red\">酶</span>稳定性和活性高、简单易操作、能够与多种检测方式联用等优点，对于发展高效快速的蛋白质组学分析方法具有重要意义。本文就<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span><span style=\"color:red\">反应器</span>的制备方法及其在蛋白质组学中的应用做简单的概述，着重介绍<span style=\"color:red\">酶</span>的<span style=\"color:red\">固定</span>方法、<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span>的载体、用于<span style=\"color:red\">固定</span>的<span style=\"color:red\">酶</span>的种类。近几年<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span><span style=\"color:red\">反应器</span>的研究集中于提高固<span style=\"color:red\">酶</span>量、保持<span style=\"color:red\">酶</span>活性、增加<span style=\"color:red\">酶</span>解效率、减小非特异性吸附等方面。研究结果表明，采用纳米材料、整体材料等新型载体，提高载体亲水性，采用多<span style=\"color:red\">酶</span>同时<span style=\"color:red\">酶</span>解等方法能够有效改善<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span><span style=\"color:red\">反应器</span>的性能，提高蛋白质的鉴定效率。","authors":[{"authorName":"张凌怡","id":"5052f220-d906-4616-aa44-f86ef465a3f2","originalAuthorName":"张凌怡"},{"authorName":"王兵兵","id":"e88c90af-98cf-463f-b09f-af728c62aebd","originalAuthorName":"王兵兵"},{"authorName":"上官露露","id":"bea002f3-2361-4536-a7f2-8a37562816b0","originalAuthorName":"上官露露"},{"authorName":"张润生","id":"34654153-1198-4d15-8696-20a7e98c1b84","originalAuthorName":"张润生"},{"authorName":"陈建虎","id":"b250b046-aa62-4cff-8124-b3f54495d391","originalAuthorName":"陈建虎"},{"authorName":"张维冰","id":"0041fe05-79da-430c-b57d-7a72040f6cae","originalAuthorName":"张维冰"}],"doi":"10.3724/SP.J.1123.2015.06028","fpage":"1121","id":"cfcc1657-db73-4cdc-8513-c95177078d49","issue":"11","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"8f297b7b-d24b-4cf0-a41d-196225fea6d6","keyword":"<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span><span style=\"color:red\">反应器</span>","originalKeyword":"固定化酶反应器"},{"id":"dd5e6a8e-982d-4fa9-a6ce-b29b892b2910","keyword":"<span style=\"color:red\">酶</span>","originalKeyword":"酶"},{"id":"1a079857-cc36-499a-9241-cf8918123de1","keyword":"载体材料","originalKeyword":"载体材料"},{"id":"fdfab1d1-90a1-4ec8-bfd7-b4b247ccbea5","keyword":"蛋白质组学","originalKeyword":"蛋白质组学"},{"id":"821b3ca2-1f85-4e04-9360-3961cc056099","keyword":"制备","originalKeyword":"制备"}],"language":"zh","publisherId":"sp201511004","title":"蛋白质组学中的<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span><span style=\"color:red\">反应器</span>制备的研究进展","volume":"","year":"2015"},{"abstractinfo":"建立了一种新型的<span style=\"color:red\">酶</span><span style=\"color:red\">固定</span><span style=\"color:red\">化</span>方法,采用DNA链置换<span style=\"color:red\">反应</span>成功地在单链DNA标记的磁性纳米粒子上实现了<span style=\"color:red\">酶</span>的链置换无损更替.该技术可实现目标<span style=\"color:red\">酶</span>的再利用,节约了生产成本.制备的<span style=\"color:red\">固定</span><span style=\"color:red\">化</span>胰蛋白<span style=\"color:red\">酶</span>微<span style=\"color:red\">反应器</span>具有较好的重复利用性和高<span style=\"color:red\">酶</span>切效率,重复使用10次后仍可保持原<span style=\"color:red\">酶</span>活性的86%;利用链置换<span style=\"color:red\">反应</span>制备的MNPs@DNA-Trypsin<span style=\"color:red\">酶</span>切马心肌红蛋白5 min后,即可获得95%±0%(n=3)的氨基酸序列覆盖率,远超过相同条件下自由<span style=\"color:red\">酶</span><span style=\"color:red\">酶</span>切12 h的结果.实验表明,发展的<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span>技术具有高磁响应性,便于从<span style=\"color:red\">反应</span>体系中回收<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span>和重复使用,同时此技术可显著提高<span style=\"color:red\">酶</span>活性,因此可用于<span style=\"color:red\">固定</span>各种重要的<span style=\"color:red\">酶</span>,同时可将其广泛应用于各种<span style=\"color:red\">酶</span>促<span style=\"color:red\">反应</span>中.","authors":[{"authorName":"宋佳一","id":"37a6c07f-bd20-4958-8200-0b420d81badf","originalAuthorName":"宋佳一"},{"authorName":"苏萍","id":"425bf4f5-1116-4a64-a62b-0088aadafc49","originalAuthorName":"苏萍"},{"authorName":"杨烨","id":"8256fb40-ccd9-4d97-8c38-765f8a457841","originalAuthorName":"杨烨"},{"authorName":"杨屹","id":"1acec5b0-6732-4ac0-910c-5747613182e7","originalAuthorName":"杨屹"}],"doi":"10.3724/SP.J.1123.2016.10023","fpage":"260","id":"7b35443b-7af0-4bf4-80bd-e8b13b7fd1b1","issue":"3","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"4bfeea95-5622-4ffb-9d16-e45d53a8ef92","keyword":"DNA链置换<span style=\"color:red\">反应</span>","originalKeyword":"DNA链置换反应"},{"id":"076b3c38-55e4-46e9-991a-b78e0d0211cb","keyword":"<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span>","originalKeyword":"固定化酶"},{"id":"fcb01356-e00a-4f90-8062-ce97de28066b","keyword":"<span style=\"color:red\">酶</span>切","originalKeyword":"酶切"},{"id":"f22dd3bb-89b1-4b7d-a86b-fbc5936b4b92","keyword":"高效液相色谱","originalKeyword":"高效液相色谱"},{"id":"eb29f621-513b-4bfc-80ab-9fe7d55a7e81","keyword":"质谱","originalKeyword":"质谱"}],"language":"zh","publisherId":"sp201703007","title":"基于DNA链置换<span style=\"color:red\">反应</span>的新型<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span><span style=\"color:red\">反应器</span>制备及应用","volume":"35","year":"2017"},{"abstractinfo":"研究了以不同粒径的磁性颗粒为载体的<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span><span style=\"color:red\">反应器</span>在蛋白质<span style=\"color:red\">酶</span>解过程中,其粒径大小对团聚、<span style=\"color:red\">酶</span>解效率和漏切位点等的影响.实验结果表明,纳米级颗粒的<span style=\"color:red\">酶</span>负载量为亚微米级的3.5倍左右.但当<span style=\"color:red\">酶</span><span style=\"color:red\">固定</span>量相同时,<span style=\"color:red\">酶</span>解效率基本相当.而在一定程度上加大磁性颗粒的粒径后,团聚现象得到明显改善.选择磁性载体粒径为20nm的<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span><span style=\"color:red\">反应器</span>,对其性能进一步考察.结果显示胰蛋白<span style=\"color:red\">酶</span>与牛血清白蛋白(BSA)的质量比为1∶1时,即能于1 min内实现快速<span style=\"color:red\">酶</span>解；当<span style=\"color:red\">酶</span>解10 min时,其零漏切位点肽段数和蛋白质序列覆盖率基本达到稳定,并明显优于溶液<span style=\"color:red\">酶</span>解水平.通过对漏切位点的统计分析比较,发现<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span>解与溶液<span style=\"color:red\">酶</span>解时的漏切位点规律基本类似.因此,采用不同粒径磁性载体制备的<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span><span style=\"color:red\">反应器</span>均可在蛋白质组学研究中提供快速、高效的<span style=\"color:red\">酶</span>解.","authors":[{"authorName":"张姣","id":"80a80c8a-57f3-487f-a279-ad037082bb34","originalAuthorName":"张姣"},{"authorName":"周廉淇","id":"76898127-77c0-41db-b444-764a61f05d38","originalAuthorName":"周廉淇"},{"authorName":"田芳","id":"c0809336-d817-46c2-9bf6-3c9de94156db","originalAuthorName":"田芳"},{"authorName":"张养军","id":"60c1240a-40b3-4c5b-9541-de7d36967a4d","originalAuthorName":"张养军"},{"authorName":"钱小红","id":"95e3b963-0d4e-4944-8535-0bc3f3f8bba9","originalAuthorName":"钱小红"}],"doi":"10.3724/SP.J.1123.2012.10019","fpage":"102","id":"c26d5abe-95e0-480e-a6b0-c24d3ecbe4bc","issue":"2","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"71637944-569a-4d50-b596-c591f8f04507","keyword":"磁性颗粒","originalKeyword":"磁性颗粒"},{"id":"d8c6c768-00bc-44e4-aed3-c92510908028","keyword":"<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span><span style=\"color:red\">反应器</span>","originalKeyword":"固定化酶反应器"},{"id":"07c47ad7-ad7e-49d7-8c3f-0e55c3478e14","keyword":"蛋白质组学","originalKeyword":"蛋白质组学"},{"id":"e09ecf5c-4ac6-4089-8945-1d266a30caa1","keyword":"蛋白质<span style=\"color:red\">酶</span>解","originalKeyword":"蛋白质酶解"}],"language":"zh","publisherId":"sp201302003","title":"采用磁性载体制备的<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span><span style=\"color:red\">反应器</span>的载体粒径对蛋白质<span style=\"color:red\">酶</span>解性能的影响","volume":"31","year":"2013"},{"abstractinfo":"综述了在聚合物膜上<span style=\"color:red\">固定</span><span style=\"color:red\">化</span>脂肪<span style=\"color:red\">酶</span>的方法及脂肪<span style=\"color:red\">酶</span>膜<span style=\"color:red\">反应器</span>的应用.重点讨论了纤维素膜、聚丙烯腈膜和聚砜膜表面的化学改性途径及其与脂肪<span style=\"color:red\">酶</span>的偶联方法.","authors":[{"authorName":"彭立凤","id":"69498792-252b-4041-bbb3-0e0dc7cc5896","originalAuthorName":"彭立凤"},{"authorName":"刘新喜","id":"b3c1f930-5d7d-450f-b207-15c01ecb3e5b","originalAuthorName":"刘新喜"},{"authorName":"杨国营","id":"8c830e73-19f4-4a5e-a26f-83fd85755a3b","originalAuthorName":"杨国营"},{"authorName":"高文玲","id":"8085fcf2-b67d-4f73-befc-52d1b55a954b","originalAuthorName":"高文玲"},{"authorName":"谭天伟","id":"c151a0b0-4dd4-4226-8e31-4bd173aca6f0","originalAuthorName":"谭天伟"}],"doi":"10.3969/j.issn.1007-8924.2001.02.011","fpage":"44","id":"0891ac5f-8ecc-490f-a2e3-f32d54d85acf","issue":"2","journal":{"abbrevTitle":"MKXYJS","coverImgSrc":"journal/img/cover/MKXYJS.jpg","id":"54","issnPpub":"1007-8924","publisherId":"MKXYJS","title":"膜科学与技术   "},"keywords":[{"id":"f43fb32e-84a9-4eee-bcd1-3a8aea7f0d8d","keyword":"膜","originalKeyword":"膜"},{"id":"21013bca-cb7e-478a-bea5-deaba369353b","keyword":"膜<span style=\"color:red\">反应器</span>","originalKeyword":"膜反应器"},{"id":"7321990d-975c-423d-92f1-5ed7aca64557","keyword":"脂肪<span style=\"color:red\">酶</span>","originalKeyword":"脂肪酶"},{"id":"e9043fe5-8217-4737-8fbf-66f03fa1edf1","keyword":"<span style=\"color:red\">酶</span><span style=\"color:red\">反应器</span>","originalKeyword":"酶反应器"}],"language":"zh","publisherId":"mkxyjs200102011","title":"<span style=\"color:red\">固定</span><span style=\"color:red\">化</span>脂肪<span style=\"color:red\">酶</span>聚合物膜的改性及脂肪<span style=\"color:red\">酶</span>膜<span style=\"color:red\">反应器</span>的应用","volume":"21","year":"2001"},{"abstractinfo":"针对传统溶液<span style=\"color:red\">酶</span>解存在的<span style=\"color:red\">酶</span>解时间较长、<span style=\"color:red\">酶</span>自切物干扰以及蛋白<span style=\"color:red\">酶</span>不能重复使用等缺陷,通过电子转移生成催化剂的原子转移自由基聚合法修饰银丝,并以其为载体制备了一种新型的<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span><span style=\"color:red\">反应器</span>.用质谱考察了银丝<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span><span style=\"color:red\">反应器</span>(SW-Trypsin)的<span style=\"color:red\">酶</span>解效率、重复性和回收率.结果表明:绒毛状聚合物修饰的SW-Trypsin的<span style=\"color:red\">酶</span>解效率较高,<span style=\"color:red\">酶</span>解标准蛋白牛血清白蛋白(BSA)20 min后,肽段的氨基酸序列覆盖率可达93％,高于传统溶液<span style=\"color:red\">酶</span>解方法<span style=\"color:red\">酶</span>解16h所得79％的覆盖率.使用该<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span><span style=\"color:red\">反应器</span>于一个月内8次<span style=\"color:red\">酶</span>解BSA所得的氨基酸序列覆盖率在89％到97％之间,平均覆盖率为94％,显示出良好的稳定性.另外,该<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span><span style=\"color:red\">反应器</span><span style=\"color:red\">酶</span>解马心肌红蛋白(MYO)的回收率为87.67％.最后,用SW-Trypsin<span style=\"color:red\">酶</span>解腾冲嗜热菌全蛋白20 min,所鉴定到的氨基酸序列覆盖率和蛋白数量与同样条件下溶液<span style=\"color:red\">酶</span>解16h的结果接近,且零漏切位点肽段的比例更高.加之容易分离的优点,SW-Trypsin在蛋白质组学的应用中具有良好的前景.","authors":[{"authorName":"周廉淇","id":"58bb47f7-2975-4210-82da-5788cc2fed2d","originalAuthorName":"周廉淇"},{"authorName":"张姣","id":"f9797cac-157e-43c1-a485-f9f579682ae4","originalAuthorName":"张姣"},{"authorName":"田芳","id":"4e26be6b-7702-471f-9987-abcdba758cb7","originalAuthorName":"田芳"},{"authorName":"张养军","id":"11b4172b-4e89-4571-94e3-a25cb69ca177","originalAuthorName":"张养军"},{"authorName":"钱小红","id":"35aab789-e051-4e0b-9c8c-03ec3594ab4e","originalAuthorName":"钱小红"}],"doi":"10.3724/SP.J.1123.2012.10030","fpage":"355","id":"6789da8f-ae6a-46f9-90a0-b95c7642275b","issue":"4","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"fff1ab59-d8f6-446e-8e3a-5ab99b5a0374","keyword":"原子转移自由基聚合<span style=\"color:red\">反应</span>","originalKeyword":"原子转移自由基聚合反应"},{"id":"14d1143d-e645-42d9-9a36-e60e8f809fab","keyword":"蛋白质组学","originalKeyword":"蛋白质组学"},{"id":"3b17bf12-522c-43be-a5fd-95bdccd38cbb","keyword":"质谱","originalKeyword":"质谱"},{"id":"3cf9b5f9-3e30-45cc-b786-41ed6b471a57","keyword":"<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span><span style=\"color:red\">反应器</span>","originalKeyword":"固定化酶反应器"},{"id":"8ff5bb57-7566-4bd6-822b-b0752972c150","keyword":"银丝","originalKeyword":"银丝"}],"language":"zh","publisherId":"sp201304013","title":"原子转移自由基聚合修饰银丝<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span><span style=\"color:red\">反应器</span>的制备及在蛋白质组鉴定中的应用","volume":"31","year":"2013"},{"abstractinfo":"以氯乙酰化聚苯乙烯微球载体为大分子引发剂,以甲基丙烯酸缩水甘油酯和亲水性丙烯酰胺为共聚接枝单体,以氯化亚铜及2,2'-联吡啶为催化体系,采用原子转移自由基聚合法接枝合成了具有柔性链的<span style=\"color:red\">亲</span><span style=\"color:red\">水</span>梳状环氧聚合物载体PS-acyl-P(AM-co-GMA),并将其用于耐有机溶剂YCJ01脂肪<span style=\"color:red\">酶</span>的共价柔性<span style=\"color:red\">固定</span><span style=\"color:red\">化</span>.结果表明,<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span>催化对硝基苯酚棕榈酸酯水解的最适温度为45～55℃,pH=8.5,此时<span style=\"color:red\">酶</span>活为16.6 U/g;在80℃以下,pH=5～10时,<span style=\"color:red\">酶</span>的活性稳定；连续进行7次间歇操作,<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span>保留初始活性的73.5％；于4℃保存60 d,<span style=\"color:red\">酶</span>活仍剩余86.8％.经<span style=\"color:red\">亲</span><span style=\"color:red\">水</span>梳状环氧载体柔性<span style=\"color:red\">固定</span><span style=\"color:red\">化</span>后,该脂肪<span style=\"color:red\">酶</span>对有机溶剂的耐受性有所提高.","authors":[{"authorName":"曲伟光","id":"c029595c-4528-4ee4-a753-b3f8d1661c67","originalAuthorName":"曲伟光"},{"authorName":"魏荣卿","id":"6e8308be-f789-4396-ae72-49ebc6fe91ba","originalAuthorName":"魏荣卿"},{"authorName":"何冰芳","id":"0b522c1a-e835-4905-9bba-360bed54a804","originalAuthorName":"何冰芳"},{"authorName":"刘晓宁","id":"f41c6cac-aa82-4f08-9f67-7bded293c560","originalAuthorName":"刘晓宁"},{"authorName":"仲玉","id":"1d5b0735-3dfb-4837-a08c-5d3ce3d21c10","originalAuthorName":"仲玉"},{"authorName":"陈新营","id":"03be0191-75df-43a8-9ead-a19367536472","originalAuthorName":"陈新营"}],"doi":"10.3724/SP.J.1088.2011.10536","fpage":"1869","id":"803fa375-d0a8-4c6b-9bcb-5dfdc42bc1c2","issue":"12","journal":{"abbrevTitle":"CHXB","coverImgSrc":"journal/img/cover/CHXB.jpg","id":"18","issnPpub":"0253-9837","publisherId":"CHXB","title":"催化学报   "},"keywords":[{"id":"81c8bdb5-d233-4f55-b8bf-82a8080110a5","keyword":"氯乙酰化聚苯乙烯","originalKeyword":"氯乙酰化聚苯乙烯"},{"id":"2e94788f-28ac-492d-af8e-c7f850d9b94b","keyword":"原子转移自由基聚合","originalKeyword":"原子转移自由基聚合"},{"id":"6ea82981-45af-4f04-af4e-972f4d4827e0","keyword":"梳状环氧聚合物","originalKeyword":"梳状环氧聚合物"},{"id":"d366ba97-4bb4-45ad-aab2-f36c5ade50db","keyword":"耐有机溶剂脂肪<span style=\"color:red\">酶</span>","originalKeyword":"耐有机溶剂脂肪酶"},{"id":"23fb60e8-9d5c-4afd-bdf0-56a0c8e34dac","keyword":"柔性<span style=\"color:red\">固定</span><span style=\"color:red\">化</span>","originalKeyword":"柔性固定化"}],"language":"zh","publisherId":"cuihuaxb201112014","title":"<span style=\"color:red\">亲</span><span style=\"color:red\">水</span>梳状环氧聚合物载体柔性<span style=\"color:red\">固定</span><span style=\"color:red\">化</span>脂肪<span style=\"color:red\">酶</span>","volume":"32","year":"2011"},{"abstractinfo":"概述了<span style=\"color:red\">酶</span>膜<span style=\"color:red\">反应器</span>的组成、分类、操作方式、<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span>的方法等,综述了国内外<span style=\"color:red\">酶</span>膜<span style=\"color:red\">反应器</span>在淀粉糖生产中的研究进展,提出了<span style=\"color:red\">酶</span>膜<span style=\"color:red\">反应器</span>应用于淀粉糖工业化生产需进一步解决的问题.","authors":[{"authorName":"李琼","id":"e3ed9c32-68e9-484b-8164-4452488fcfd8","originalAuthorName":"李琼"},{"authorName":"肖晶","id":"5539ac1d-71bf-4578-b64c-261751f10425","originalAuthorName":"肖晶"},{"authorName":"刘毓宏","id":"7774dd7e-a5f6-4831-bd83-e0f03ec5b200","originalAuthorName":"刘毓宏"}],"doi":"10.3969/j.issn.1007-8924.2004.02.014","fpage":"58","id":"08362d8a-b076-4cd6-a17d-03d41c45188e","issue":"2","journal":{"abbrevTitle":"MKXYJS","coverImgSrc":"journal/img/cover/MKXYJS.jpg","id":"54","issnPpub":"1007-8924","publisherId":"MKXYJS","title":"膜科学与技术   "},"keywords":[{"id":"c9fdbdb9-c351-40c7-a3c7-9e13fcffb2bb","keyword":"<span style=\"color:red\">酶</span>膜<span style=\"color:red\">反应器</span>","originalKeyword":"酶膜反应器"},{"id":"e6466302-3ea5-44b3-ad3c-580ed3b6510e","keyword":"淀粉糖","originalKeyword":"淀粉糖"},{"id":"5ad63176-f57a-4275-b46b-caae0a9b21ae","keyword":"生产","originalKeyword":"生产"}],"language":"zh","publisherId":"mkxyjs200402014","title":"<span style=\"color:red\">酶</span>膜<span style=\"color:red\">反应器</span>及其在淀粉糖中的应用","volume":"24","year":"2004"},{"abstractinfo":"采用原子转移自由基聚合法制备了<span style=\"color:red\">亲</span><span style=\"color:red\">水</span>聚合物修饰的硅胶颗粒作为一种新型<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span>载体,在实现胰蛋白<span style=\"color:red\">酶</span>高密度<span style=\"color:red\">固定</span>的同时,显著降低了载体材料非特异性吸附导致的样品损失.因此,该<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span>材料兼具高<span style=\"color:red\">酶</span>解效率和高回收率的特性.以标准蛋白质牛血清白蛋白(BSA)为样本,使用该<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span>1 mm即可完成<span style=\"color:red\">酶</span>解,鉴定到肽段对BSA的氨基酸序列覆盖率可达90％以上.该<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span>材料成功应用于酵母菌全蛋白质复杂样本的<span style=\"color:red\">酶</span>解,从3 min<span style=\"color:red\">酶</span>解产物中鉴定到666个蛋白质,超过同样条件下溶液<span style=\"color:red\">酶</span>解12 h的鉴定结果.","authors":[{"authorName":"范超","id":"d5ba565d-a887-4a36-b4f5-4fd8c1f03775","originalAuthorName":"范超"},{"authorName":"宋子凤","id":"1e174e72-6a6c-41dc-9823-80f5b1b7b3ff","originalAuthorName":"宋子凤"},{"authorName":"秦伟捷","id":"0afc49a1-1f3d-4585-a851-a2686142c261","originalAuthorName":"秦伟捷"},{"authorName":"蔡耘","id":"f5aaa770-ddf3-4222-907f-4e3cbb46681f","originalAuthorName":"蔡耘"},{"authorName":"钱小红","id":"ef91c47a-c1b9-489d-932d-d84717e64ac6","originalAuthorName":"钱小红"}],"doi":"10.3724/SP.J.1123.2013.01007","fpage":"423","id":"df3b00ce-dc07-4629-aa48-0dd07f661371","issue":"5","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"71bcd67c-e2a4-419c-afac-babf6c080f2e","keyword":"<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span>","originalKeyword":"固定化酶"},{"id":"24e4cb61-fe44-4d36-a21c-5f3d3969b837","keyword":"蛋白质组学","originalKeyword":"蛋白质组学"},{"id":"8123dee0-810c-4b11-8128-01b23fa360c8","keyword":"原子转移自由基聚合<span style=\"color:red\">反应</span>","originalKeyword":"原子转移自由基聚合反应"}],"language":"zh","publisherId":"sp201305005","title":"新型<span style=\"color:red\">亲</span><span style=\"color:red\">水</span>聚合物修饰硅胶颗粒<span style=\"color:red\">固定</span><span style=\"color:red\">化</span><span style=\"color:red\">酶</span>的制备及在蛋白质组鉴定中的应用","volume":"31","year":"2013"},{"abstractinfo":"对<span style=\"color:red\">酶</span>膜<span style=\"color:red\">反应器</span>的国内外研究现状和发展趋势进行简要介绍.概述了<span style=\"color:red\">酶</span>膜<span style=\"color:red\">反应器</span>的特点及其分类,介绍了<span style=\"color:red\">酶</span>膜<span style=\"color:red\">反应器</span>的应用领域和一些应用实例.讨论了<span style=\"color:red\">酶</span>膜<span style=\"color:red\">反应器</span>目前存在的一些瓶颈问题及其解决办法.","authors":[{"authorName":"姜忠义","id":"aeb0e373-c0fc-4009-ae14-4065815365b0","originalAuthorName":"姜忠义"},{"authorName":"陈洪钫","id":"c8dc9f81-a595-4c18-9a91-92a7aa606938","originalAuthorName":"陈洪钫"}],"doi":"","fpage":"14","id":"9ce46a0d-ec5c-4829-8c6a-88fa66a6f312","issue":"1","journal":{"abbrevTitle":"GFZCLKXYGC","coverImgSrc":"journal/img/cover/GFZCLKXYGC.jpg","id":"31","issnPpub":"1000-7555","publisherId":"GFZCLKXYGC","title":"高分子材料科学与工程"},"keywords":[{"id":"22395f58-7c51-435c-b25f-92b5e7e5f7c3","keyword":"<span style=\"color:red\">酶</span>膜<span style=\"color:red\">反应器</span>","originalKeyword":"酶膜反应器"},{"id":"af5035ff-c32e-4164-bd71-f00e5a859f66","keyword":"分类","originalKeyword":"分类"},{"id":"839c64d3-290c-4953-8653-f867fc270a31","keyword":"特点","originalKeyword":"特点"},{"id":"af8e3945-b055-40ec-9e68-355e96b34d2e","keyword":"应用","originalKeyword":"应用"},{"id":"9c615388-4fd2-41fd-a062-b874a2f7eb6a","keyword":"问题","originalKeyword":"问题"},{"id":"19bbbf33-109d-49d9-83f2-f146b4394cd2","keyword":"对策","originalKeyword":"对策"}],"language":"zh","publisherId":"gfzclkxygc200401004","title":"<span style=\"color:red\">酶</span>膜<span style=\"color:red\">反应器</span>研究进展","volume":"20","year":"2004"}],"totalpage":7934,"totalrecord":79334}